周忠;陈默;井晓婷;严英;万雅璐;

• 1:贵阳市公共卫生救治中心呼吸与危重症学科

摘要(Abstract)：

目的 分析重症肺炎(SP)患者蛋白激酶B(Akt)/核转录因子κB(NF-κB)信号通路分子表达变化及其与预后的关系。方法 选取2020年3月-2022年3月贵阳市公共卫生救治中心收治的82例SP患者为研究组，另选取同期82例普通肺炎患者为对照组。采用荧光定量PCR检测Akt、NF-κB mRNA相对表达量，酶联免疫吸附法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6水平，绘制受试者工作特征(ROC)曲线分析Akt/NF-κB信号通路分子表达对SP患者预后的预测价值。结果 研究组Akt、NF-κB mRNA相对表达量，TNF-α、IL-1β、IL-6水平均高于对照组(P<0.05); 82例SP患者入院后28 d死亡30例，死亡组患者Akt、NF-κB mRNA相对表达量及炎症因子TNF-α、IL-1β、IL-6水平均高于存活组(P<0.05); Pearson相关性分析显示，Akt、NF-κB mRNA与TNF-α、IL-1β、IL-6水平呈正相关(P<0.05); ROC曲线分析显示，Akt、NF-κB mRNA相对表达量联合预测SP患者预后的曲线下面积(AUC)为0.869,敏感度为96.67%,特异度为63.46%。结论 SP患者血清Akt/NF-κB信号通路分子表达上调，检测Akt/NF-κB信号通路分子表达对预后具有良好的预测价值。

关键词(KeyWords)： 重症肺炎;蛋白激酶B/核转录因子κB信号通路;炎症因子;预后;预测价值

Abstract:

Keywords:

基金项目(Foundation): 贵州省科研基金资助项目(2020G021)

作者(Authors): 周忠;陈默;井晓婷;严英;万雅璐;

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