王沂霞,闫敏,吴涛,张超,屈文静,李祥

• 1:济南市第二妇幼保健院儿科
• 2:济南市第二妇幼保健院儿科急诊

摘要(Abstract)：

目的 分析2022-2025年于济南市第二妇幼保健院就诊的感染性腹泻儿童中分离的沙门氏菌血清学分型及毒力基因特征。方法 纳入2022年1月-2025年2月济南市第二妇幼保健院儿科收治的620例感染性腹泻儿童，应用传统分离培养与荧光定量聚合酶链式反应（q-PCR）技术对其粪便进行沙门氏菌分离培养鉴定，并应用PCR技术对分离菌进行血清分型及毒力基因分型。分析感染性腹泻儿童临床特征、沙门氏菌阳性病例的季节及时间分布、沙门氏菌血清型构成比、沙门氏菌耐药性、耐药谱及毒力基因携带情况。结果 620例感染性腹泻患儿中男孩占61.77%(383/620)，年龄段以6个月～2岁居多58.87%(365/620)，以水样便性状居多60.16%(373/620)，其他症状腹痛居多37.10%(230/620)，沙门氏菌阳性检出率为7.58%(47/620)；沙门氏菌在不同地区不同季节呈现血清型分布差异性，本地区夏秋季（尤其是7-9月）为检出高峰；47株沙门氏菌血清型以鼠伤寒沙门氏菌居多46.81%(22/47)，其次为肠炎沙门氏菌17.02%(8/47)，其余血清型较少；47株沙门氏菌对四环霉素，氨苄西林，阿奇霉素，氯霉素的耐药率均> 50%，对其他抗菌药物的耐药率较低；39株（82.98%）沙门氏菌呈多重耐药；47株沙门氏菌携带的毒力基因中gip A、avr A、ssa Q、mgt C、sod C1携带率为100.00%，其次为spo B、ssi D、spo E，分别为93.62%、91.49%、85.11%,spv C较少，仅23.40%。结论 本中心感染性腹泻儿童分离的沙门氏菌以鼠伤寒沙门氏菌居多，对四环霉素、氨苄西林耐药率较高，毒力基因携带率较高，临床需重视并采取预防措施。

关键词(KeyWords)： 感染性腹泻;沙门氏菌;血清学分型;毒力基因;耐药性;儿童

Abstract:

Keywords:

基金项目(Foundation): 济南市卫生健康委员会科技计划项目（2022-2-139）

作者(Author): 王沂霞,闫敏,吴涛,张超,屈文静,李祥

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